NIOS Class 12 Biology 314 Solved Practical File (Lab Manual) - Latest Edition October 2025
Download the latest version of NIOS Class 12 Biology 314 Lab Manual (Solved Practical File)
The biology practicals are more than just a formality. They're an essential part of the NIOS Class 12 science curriculum. Each year, thousands lose marks because their practical files were incomplete, incorrect, or did not meet board standards. The Unnati Education NIOS Class 12 Biology 314 Solved Lab Manual is the most reliable and syllabus-based resource that you will find in 2025.
We know the challenges NIOS students are facing: limited practical guidance, unclear instructions, and a lack of examples. We've developed a ready-to-submit, fully solved Biology practical file that is aligned with the latest NIOS guidelines. This will ensure your submission and marks are maximized.
Table of Contents
| S. No. | Name of the Exercise | Page No. |
|---|---|---|
| 1 | Introduction | 1 |
| 2 | Exercise 1: Some Common Instruments | 5 |
| 3 | Exercise 2: | |
| 3.1 | Preparation of a temporary mount of onion peel to observe and study epidermal cells | 11 |
| 3.2 | Preparation of a temporary stained mount of human cheek cells | 16 |
| 3.3 | Preparation of a temporary mount of leaf epidermis to study the structure of stomata | 19 |
| 3.4 | Preparation and study of xylem and phloem from the Cucurbita stem | 22 |
| 3.5 | Temporary stained preparation and study of striated muscle fibres in the cockroach | 24 |
| 4 | Exercise 3: Study of morphological modifications of plant parts like root, stem, and leaf | 27 |
| 5 | Exercise 4: To study the T.S. of dicot and monocot stems and roots from a permanent slide | 36 |
| 6 | Exercise 5: Study of microscopic anatomy (histology) of mammalian tissues and organs | 45 |
| 7 | Exercise 6: To study the structure and function of different parts of flowers (China rose and Petunia) | 52 |
| 8 | Exercise 7: Study of animal specimens and their classification (e.g., sponge, earthworm, butterfly, apple snail, starfish, dogfish, Rohu, toad, lizard, pigeon, bat) | 60 |
| 9 | Exercise 8: Preparation of a slide of onion root tip for observation of mitosis stages | 81 |
| 10 | Exercise 9: To study the special adaptive features in some plants and animals | 88 |
| 11 | Exercise 10: (a) Physical properties of different soil samples | 93 |
| 11.2 | (b) Water holding capacity of different soils | 96 |
| 12 | Exercise 11: Demonstration of osmosis by potato osmometer | 100 |
| 13 | Exercise 12: Determine the rate of photosynthesis in an aquatic plant (Hydrilla or Elodea) | 102 |
| 14 | Exercise 13: Study the structure and germination in gram and bean seeds | 105 |
| 15 | Exercise 14: Demonstrate the release of COâ‚‚ during seed germination | 111 |
| 16 | Exercise 15: Study the action of salivary amylase on starch | 114 |
| 17 | Exercise 16 (Optional Module): | 119 |
| 17.1 | Study the developmental stages in the life cycle of Drosophila by preparing a culture | 122 |
| 17.2 | Project on the growth pattern of money plant | 124 |
| 17.3 | Preparation of a herbarium | 124 |
Download Solved Practical File
The NIOS Class 12 Biology 314 Solved Lab Manual (Lab Manual) is essential for every student
30%-40% of the total marks are attributed to the practical section. You must demonstrate your hands-on knowledge. It is not enough to just memorize the theory. The NIOS Biology Practical File class 12 is an official document that certifies your Lab knowledge. It covers experiments related to animals, plants, and human biology.
However, manually creating this file can be stressful. Students often struggle with what to include, how to format diagrams, or how to write observations correctly. One mistake can result in lower scores or rejection from the study center.
This risk is eliminated by our NIOS Class 12 Biology 314 solved practical file (Lab Manual). Each page is laid out according to the NIOS-approved layout, with a precise aim, material lists, step-by-step procedures, observations, results, and conclusions, all neatly written for direct submission.
What's in the latest NIOS Biology practical file for Class 12?
This updated lab manual provides you with a conceptual understanding of all the key Biology experiments that you will perform in your NIOS course. The practical file includes:
- Cell structure and microscopy: Includes exercises on onion peels, human cheek cells, and plant stomata, teaching how to identify cell components under a microscope.
- Plant Anatomy & Physiology: Exercises on stems of dicots and monocots, transpiration, and osmosis. Photosynthesis and seed germination are also included.
- Human Anatomy and Animal Anatomy. Histology, mammalian system, and muscle fibres.
- Genetics & Growth Studies: Mitotic Division in Onion Root Tip, Drosophila developmental stages, and plant growth patterns.
- Environmental and Ecological Experiments: Analysis of soil properties, water holding capacity, and adaptations of plants and animals.
The observation table and expected result are clearly explained for each topic. This makes it easier to learn the material and confidently present yourself during a viva.
Why students choose Unnati Education's Biology Lab Manual
- Alignment with the latest 2025 NIOS Guidelines
This lab manual has been fully revised to match the October 2025 NIOS exam. It ensures that your file is in line with the current syllabus, scoring scheme, and board submission formats. - It is easy to understand and exam-oriented
Each experiment has a logical format: Aim, Apparatus, Procedures, and Observations.. The book is written in simple language for home-study and self-learning students. - Diagrams and explanations that are accurate
The diagrams are neatly labeled and illustrated according to NIOS standards. This makes your file ready for presentation. - Available in Hindi and English Medium
Students of all mediums can prepare for the exam by using our NIOS Biology 314 Solved Lab Manual (Lab Manual). - Choose between a PDF or a handwritten file
Instant digital download (PDF) and a professionally printed handwritten file are available for submission.
Who can benefit from this Solved Practical File?
This NIOS Biology class 12 practical file is ideal for:
- Students who are enrolled in stream 1, 2, 3, or 4.
- Students who have missed previous practicals can return to class or reappear in their respective compartments.
- Students who study through distance learning or open schooling
- Working professionals who complete their education
- Students abroad (UAE, Nepal, Saudi Arabia, etc.) Preparing for Online Submission
This resource will save you hours if you have missed practical classes or have limited time to finish your file.
The Solved Practical File helps you score better
- Saves time: You don't need to format everything from scratch.
- Increases Confidence: You will know what your examiner is checking.
- Avoids Mistake: Includes correct illustrations, terminology, and observations.
- Improves Performance in Viva Questions: Each subject includes clear conceptual explanations so that you can answer questions with confidence.
- Impress evaluators with a neat and well-structured presentation.
How to get your file in a step-by-step process
Unnati Education makes it easy to get your NIOS Class 12 Biology 314 Solved practical File (Lab Manual).
- WhatsApp us on +91 9999 436384 and let us know your subject code and name (Biology 314).
- Select your preferred format: Handwritten or PDF.
- Complete your payment using Paytm, UPI, or Bank Transfer.
- Choose for courier delivery or for instant digital delivery.
- All files have been verified, updated, and formatted according to NIOS requirements.
Unnati Education is trusted by NIOS students across India
Unnati Education is a leading provider of NIOS/IGNOU guidance for more than 10 years. Our team is made up of subject experts and academic content developers, who make sure that every practical file is clean, correct, and approved by the examiner.
We have helped thousands of students in Delhi, UP, Bihar, Maharashtra, Rajasthan, Gujarat, and other states to submit perfect files and pass exams with distinction.
We also offer:
- Solved assignments (TMA) for All Subjects
- Admission Support for Streams 1 to 4
- Previous years' question papers and sample papers
- Online Classes in Physics, Chemistry, and Biology.
- IGNOU projects, assignments, and synopses
We are known for our fast service, quality content, and academic support.
How to prepare for NIOS Biology practicals like a topper
Preparation is essential, even if you have a file that has been solved. Here are some expert tips to help you prepare for your NIOS practical test:
- Re-examine the theory that underlies each experiment, especially in terms of definitions and chemistry principles.
- You should be able to identify the diagrams and their labels.
- Understanding the results interpretation is better than memorization.
- Keep your files neat and properly stapled. Also, organize them with an index and a certificate.
- Explain the experiments to yourself before the viva.
- Before your final exam, go through the NIOS Class 12 Biology practical file viva questions.
These small steps will ensure a confident performance with maximum marks.
Final Words
The Unnati Education NIOS class 12 Biology 314 Solved practical file (Lab manual) is more than just a document--it will help you score full marks on Biology practicals. This file is perfect for students who are short on time or are unsure about the format. It contains everything they need to finish their work correctly and confidently.
We have helped thousands of students in India and overseas meet their deadlines. Your practical file will help you to prepare for the October 2025 NIOS exams.
WhatsApp Now: 9899436384 Email: info@unnatieducation.in Website: unnatieducation.in
FAQs (Frequently Asked Questions)
Q1. Why is this NIOS Class 12 biology 314 solved practical file (Lab Manual), accepted by the NIOS Board?
Yes. The files are all in the latest NIOS format 2025 and have been verified by subject experts. Thousands of students in India have successfully submitted these files at their respective study centres.
Q2. Does this practical file help with viva preparation too?
This practical file is also useful for viva preparation. An explanation of the scientific principles behind it accompanies each experiment. This will help you answer questions confidently during the viva.
Q3. Can I get the NIOS Biology Practical File in Hindi?
You can choose between the Hindi and English versions depending on your exam needs.
Q4. How can I get my file quickly before submission deadlines?
You can send us a WhatsApp message to get your file before the deadline. You can get instant PDF files and a courier service within 24 hours.
Q5. Is this file suitable for students who have failed or reappeared?
This file is perfect for students who have to reappear or missed their practical session. The file has been formatted for direct submission.