Every NIOS learner reaches a stage where the textbook feels heavy and the exam date feels uncomfortably close. That is exactly the moment a tight set of NIOS Class 12 Biology 314 Objective Type Questions starts to matter. This page hands you real sample questions with answers for the 2026 session, pulled straight from our full pack, so you can weigh the quality yourself before you ask us for the complete set.
What you see below is not filler. Each sample is a genuine objective-type question we prepared for the Biology 314 paper, with the answer and a short reason attached. Read a few, check them against your own understanding, and decide whether this is the kind of practice that will carry you through the examination.
NIOS Class 12 Biology 314 Objective Type Questions for 2026
The Biology 314 paper rewards students who practise the objective section seriously. Multiple choice items, fill in the blanks, true or false lines and match the following blocks together carry a real chunk of the paper, and they are the quickest marks to secure if you have seen the pattern before. Our set gathers the most repeated and most probable of these across every important chapter of the course.
We keep the language plain on purpose. A learner reading in English medium should be able to follow the question, understand why the answer is correct, and move on without getting stuck. If you have already started your revision, pairing this practice with the official NIOS Syllabus keeps you aligned with what the board actually expects this session.
Why these NIOS Biology 314 questions help you pass
Biology at the senior secondary level is a memory heavy subject, and the objective portion is where careful preparation shows up fastest. A student who has drilled the important terms, ratios and matching pairs walks into the hall able to finish the first section quickly and spend the saved time on the longer answers.
That is the whole design idea here. Instead of asking you to re-read three hundred pages, we surface the lines that examiners keep returning to. Diseases and their causative agents, pigments and their roles, genetic ratios, enzymes and their functions, these are the small facts that decide two and three mark objective questions. Getting them automatic is the difference between a safe pass and a nervous one.
How we built these NIOS Biology 314 objective type questions
Our team did not generate these in a hurry. We sat with the previous year question papers of Biology 314 and marked every objective item that had appeared, noting which chapters the board leans on year after year. That analysis of past papers is the backbone of the whole pack.
Next, we read the complete NIOS text for the subject, chapter by chapter, and lifted the factual points that convert cleanly into objective questions. Where a topic showed a clear examination pattern, we framed fresh questions in the same style so you practise the format, not just a fixed list. Every answer was then checked for accuracy against the textbook before it went in.
The result is a set that reflects both what has been asked and what is likely to be asked. If you are building a wider revision routine, our organised NIOS Study Material sits alongside this practice and covers the descriptive answers too.
Sample multiple choice questions from NIOS Biology 314
Here are a few multiple choice questions taken directly from our pack, drawn from different chapters so you can see the spread. Each one comes with the correct option and a one line reason, which is how every question in the full set is presented.
From Lesson 2, The Kingdoms Monera, Protoctista and Fungi
1. Which of the following structures in a bacterium is directly involved in the transfer of genetic material during conjugation?
(A) Flagellum
(B) Pili
(C) Mesosome
(D) Ribosome
Answer: (B) Pili
Explanation: Pili help in conjugation by forming a bridge between two bacteria for DNA transfer.
2. The bacterial cell wall is primarily composed of which unique compound?
(A) Cellulose
(B) Chitin
(C) Peptidoglycan
(D) Lignin
Answer: (C) Peptidoglycan
Explanation: Bacterial cell walls contain peptidoglycan, a polymer of sugars and amino acids unique to them.
From Lesson 11, Photosynthesis
3. Which one of the following processes results in the production of oxygen in photosynthesis?
(A) Photophosphorylation
(B) Photolysis of water
(C) Photorespiration
(D) Reduction of NADP+
Answer: (B) Photolysis of water
Explanation: Water is split in PSII by light (photolysis), releasing O2.
4. The specific primary electron acceptor in Photosystem II is:
(A) Ferredoxin
(B) Plastocyanin
(C) Pheophytin
(D) Plastoquinone
Answer: (C) Pheophytin
Explanation: Pheophytin is the modified chlorophyll-a molecule that accepts electrons from P680 in PSII.
Fill in the blanks practice from NIOS Biology 314
Fill in the blanks questions test whether a term is truly fixed in your memory. The samples below come from the genetics chapter, one of the highest scoring areas in the paper, and each blank is followed by the exact answer we provide in the pack.
From Lesson 22, Principles of Genetics
1. The phenomenon in which both alleles in a heterozygous condition express themselves equally is known as __________.
Answer: Codominance
2. The exchange of genetic material between non-sister chromatids of homologous chromosomes during meiosis is called __________.
Answer: Crossing over
3. In incomplete dominance, the F2 phenotypic ratio is __________, which is identical to its genotypic ratio.
Answer: 1 : 2 : 1
4. The chromosomal disorder caused by trisomy of chromosome 21 is known as __________.
Answer: Down’s syndrome
True or false statements from NIOS Biology 314
True or false lines look simple, yet they punish loose reading. A single wrong word can flip a correct sounding statement into a false one, so the pack trains you to read the whole line before you decide. A few sample statements from this section are shown below.
[DATA PLACEHOLDER: True or false statement 1 with answer]
[DATA PLACEHOLDER: True or false statement 2 with answer]
[DATA PLACEHOLDER: True or false statement 3 with answer]
Solved match the following from NIOS Biology 314
Match the following blocks reward students who know their pairs cold. Organisms with diseases, tools with functions, hormones with glands, these matching sets appear across chapters and carry easy marks once memorised. Here is one solved example from the biotechnology chapter.
From Lesson 30, Biotechnology
Match the tools of genetic engineering with their functions.
| Column A | Column B |
|---|---|
| (i) Restriction Endonuclease | (a) Result of uneven DNA cutting |
| (ii) DNA Ligase | (b) Used to carry foreign gene |
| (iii) Plasmid | (c) Cuts DNA at specific sites |
| (iv) Sticky Ends | (d) Joins DNA fragments |
Answer: (i) – (c), (ii) – (d), (iii) – (b), (iv) – (a)
Explanation: Restriction endonucleases cut DNA at specific base sequences, DNA ligase joins DNA fragments, plasmids carry foreign genes into bacteria, and sticky ends are the overhanging ends left after cutting that make ligation easier.
Preview pages from our NIOS Biology 314 question pack
The samples above are only a slice of the material. To show you the depth and the neat formatting, here are a few page previews from the actual document, so you know exactly what lands on your phone after you contact us.
Preview one, multiple choice questions with answers and explanations.
Preview two, fill in the blanks and match the following from a single chapter.
Preview three, chapter wise objective questions laid out for quick revision.
Chapter list covered in NIOS Biology 314 objective type questions
The full Biology 314 course runs to thirty one chapters across five modules. Our question pack focuses on the chapters that carry the most weight in the objective section, while the table below gives you the complete syllabus map so nothing feels hidden. Learners who also want the descriptive practice can pair this with our chapter wise NIOS Class 12 Objective Type Questions booklet.
| Lesson | Chapter name |
|---|---|
| 1 | Origin and Evolution of Life and Introduction to Classification |
| 2 | The Kingdoms Monera, Protoctista and Fungi |
| 3 | Kingdoms Plantae and Animalia |
| 4 | Cell Structure and Function |
| 5 | Tissues and Other Levels of Organization |
| 6 | Root System |
| 7 | Shoot System |
| 8 | Absorption, Transport and Water Loss in Plants |
| 9 | Nutrition in Plants and Mineral Nutrition |
| 10 | Nitrogen Metabolism |
| 11 | Photosynthesis |
| 12 | Respiration in Plants |
| 13 | Nutrition and Digestion |
| 14 | Respiration and Elimination of Nitrogenous Waste |
| 15 | Circulation of Body Fluids |
| 16 | Coordination and Control, the Nervous and Endocrine Systems |
| 17 | Homeostasis and the Steady State |
| 18 | [VERIFY] Reproduction in Plants |
| 19 | [VERIFY] Reproduction in Animals |
| 20 | [VERIFY] Growth and Development |
| 21 | Reproduction and Population Control |
| 22 | Principles of Genetics |
| 23 | Molecular Inheritance and Gene Expression |
| 24 | Genetics and Society |
| 25 | Principles of Ecology |
| 26 | Conservation and Use of Natural Resources |
| 27 | Pollution |
| 28 | Nutrition and Health |
| 29 | Some Common Human Diseases |
| 30 | Biotechnology |
| 31 | Immunobiology, an Introduction |
Students preparing across classes often ask for the lower level map as well, and our NIOS Class 10 Syllabus page lays that out clearly for anyone helping a younger sibling revise at the same time.
How to get the complete NIOS Class 12 Biology 314 Objective Type Questions
Getting the full pack is quick. Message us on WhatsApp, tell us you want the Biology 314 objective set for the 2026 session, and we send the complete chapter wise document across so you can start practising the same day. You get every multiple choice item, all the fill in the blanks, the true or false lines and the solved match the following blocks for the important chapters.
Get the full pack on WhatsApp, 9654 279 279
If you have questions before you order, or you want to know which other Biology 314 material we stock, the same number works for a quick chat. Many students also add the NIOS Previous Year Question Paper collection so they can practise full papers under timed conditions once the objective section feels solid.
Ask a question on WhatsApp, 9899 436 384
NIOS Biology 314 Objective Type Questions frequently asked
Are these NIOS Biology 314 Objective Type Questions enough to pass the exam?
These NIOS Biology 314 Objective Type Questions cover the objective section thoroughly, which is a reliable scoring area in the paper. They give you a strong base for multiple choice, fill in the blanks, true or false and match the following. For a full pass you should still practise the descriptive answers and past papers, so treat this pack as the objective backbone of your revision rather than the only resource.
Which chapters do the NIOS Biology 314 objective questions come from?
The NIOS Biology 314 objective questions are drawn from the exam heavy chapters across all five modules, including genetics, photosynthesis, ecology, biotechnology and the kingdoms of classification. The chapter table on this page shows the complete thirty one lesson syllabus so you can see the coverage. Our pack concentrates on the lessons that appear most often in the objective section of the paper.
Are the answers in the NIOS Biology 314 pack correct and verified?
Yes, every answer in the NIOS Biology 314 pack is checked against the official NIOS textbook before it goes in. We build the questions from previous year papers and the prescribed text, then verify each answer and add a short explanation where it helps. If you ever spot something that reads oddly, message us and we review it, since accuracy is the whole point of the set.
Is the NIOS Biology 314 question set updated for the 2026 session?
Yes, the NIOS Biology 314 question set is prepared for the 2026 session and reflects the current syllabus and recent paper trends. We revisit the pack against the latest patterns and official notifications so your practice stays relevant. When the board releases fresh sample papers, we update the objective questions accordingly, so you are not revising from an outdated list.
How do I receive the full NIOS Biology 314 Objective Type Questions after paying?
You receive the full NIOS Biology 314 Objective Type Questions as a document on WhatsApp soon after you confirm. Message the number on this page, ask for the Biology 314 objective set, and our team shares the complete chapter wise file so you can start the same day. Delivery is digital, which means you can revise on your phone or print the pages you want to keep beside you.
Disclaimer: Unnati Educations is an independent academic support provider and is not affiliated with, endorsed by or associated with the National Institute of Open Schooling (NIOS). All chapter names and syllabus references belong to NIOS. Our material is prepared to help students practise and revise. Sources, the official NIOS Biology 314 curriculum and course text at nios.ac.in, and analysis of previous year Biology 314 question papers.
